Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit | KTE62419

Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit | KTE62419 | Gentaur UK, US & Europe Distribution

Application: This Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit employs a two-site sandwich ELISA to quantitate CMTM6 in samples. An antibody specific for CMTM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCMTM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CMTM6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CMTM6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human CKLF-like MARVEL transmembrane domain-containing protein 6 microplate
• Human CKLF-like MARVEL transmembrane domain-containing protein 6 standard
• Human CKLF-like MARVEL transmembrane domain-containing protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) ELISA Kit has high sensitivity and excellent specificity for detection of Human CMTM6. No significant cross-reactivity or interference between Human CMTM6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Members of the chemokine-like factor superfamily, including CKLFSF6, share similarity with both the chemokine and the transmembrane 4 superfamilies of signaling molecules.By screening databases for sequences that share homology with chemokine-like factor (CKLF), followed by RT-PCR of a prostate cDNA library, Han et al. (2003) cloned CKLFSF6. The deduced 183-amino acid CKLFSF6 protein has 4 transmembrane segments. It shares about 31% sequence identity with CKLFSF4 . Northern blot analysis detected a transcript of 3.88 kb expressed in leukocytes, placenta, and testis. A smaller transcript was also detected in leukocytes. Han et al. (2003) cloned mouse Cklfsf6, which shares about 88.5% sequence similarity with CKLFSF6.

Alternative Names: CMTM6; CKLFSF6; FLJ20396; PRO2219; chemokine-like factor super family 6; chemokine-like factor superfamily 6

Search name: CMTM6; CKLFSF6; FLJ20396; PRO2219; chemokine-like factor super family 6; chemokine-like factor superfamily 6

Tag: CMTM6