Human CL (Cardiolipin) ELISA Kit | ELK4525

Human CL (Cardiolipin) ELISA Kit | ELK4525 | Gentaur UK, US & Europe Distribution

Alternative Names: 1, 3-Bis (sn-3-Phosphatidyl) -Sn-Glycerol

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.5 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Cardiovascular biology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cardiolipin (CL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cardiolipin (CL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiolipin (CL) in the samples is then determined by comparing the OD of the samples to the standard curve.