Human CLDN5 (Claudin 5) ELISA Kit | ELK4125

Human CLDN5 (Claudin 5) ELISA Kit | ELK4125 | Gentaur UK, US & Europe Distribution

Alternative Names: AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS

Species: Human

Assay Type: Sandwich

Sensitivity: 13.1 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 5 (CLDN5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the OD of the samples to the standard curve.