Human CLDN9 (Claudin 9) ELISA Kit | ELK6130

Human CLDN9 (Claudin 9) ELISA Kit | ELK6130 | Gentaur UK, US & Europe Distribution

Alternative Names: CLDN9; Claudin-9

Species: Human

Assay Type: Sandwich

Sensitivity: 33 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 9 (CLDN9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 9 (CLDN9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 9 (CLDN9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 9 (CLDN9) in the samples is then determined by comparing the OD of the samples to the standard curve.