Human CLEC11A (C-Type Lectin Domain Family 11, Member A) ELISA Kit | ELK6393

Human CLEC11A (C-Type Lectin Domain Family 11, Member A) ELISA Kit | ELK6393 | Gentaur UK, US & Europe Distribution

Alternative Names: SCGF; P47; LSLCL; CLECSF3; Stem Cell Growth Factor; Lymphocyte Secreted C-Type Lectin; C-type lectin superfamily member 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.39 ng/mL

Standard: 60 ng/mL

Detection Range: 0.94-60 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the OD of the samples to the standard curve.