Human CLEC2C (C-Type Lectin Domain Family 2, Member C) ELISA Kit | ELK3755

Human CLEC2C (C-Type Lectin Domain Family 2, Member C) ELISA Kit | ELK3755 | Gentaur UK, US & Europe Distribution

Alternative Names: CD69; CLEC2-C; AIM; p60; EA1; MLR-3; Early T-Cell Activation Antigen p60; Early activation antigen CD69; Activation inducer molecule; Leukocyte surface antigen Leu-23

Species: Human

Assay Type: Sandwich

Sensitivity: 6.1 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 2, Member C (CLEC2C) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 2, Member C (CLEC2C) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 2, Member C (CLEC2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 2, Member C (CLEC2C) in the samples is then determined by comparing the OD of the samples to the standard curve.