Human COL1a2 (Collagen Type I Alpha 2) ELISA Kit | ELK1985

Human COL1a2 (Collagen Type I Alpha 2) ELISA Kit | ELK1985 | Gentaur UK, US & Europe Distribution

Alternative Names: COL1-A2; OI4; Collagen Alpha-2 (I) chain; osteogenesis imperfecta type IV; Collagen Of Skin, Tendon And bone, Alpha-2 Chain

Species: Human

Assay Type: Sandwich

Sensitivity: 6.3 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the OD of the samples to the standard curve.