740

Human Complement factor H-related protein 1 (CFHR1) ELISA Kit | AE61460HU

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SKU:
740-AE61460HU
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€1,830.00 - €3,213.00

Description

Human Complement factor H-related protein 1 (CFHR1) ELISA Kit | AE61460HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: CFHR1

Alternative Name: CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; H36-1; H36-2; HFL1; HFL2; MGC104329; H factor (complement) -like 1|H factor (complement) -like 2|complement factor H-related 1 pseudogene

Application: ELISA

Range: 0.625-40 ng/mL

Sensitivity: 0.263 ng/mL

Intra-Assay: ≤3.5%

Inter-Assay: ≤6.9%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CFHR1 in samples. An antibody specific for CFHR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCFHR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFHR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFHR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: encodes a secreted protein belonging to the complement factor H protein family. It binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic-uremic syndrome.Factor H, via SCR domains 6 to 7 and 19 to 20, and FHR1, via SCR domains 3 to 5, bound to surface-expressed Pseudomonas aeruginosa elongation factor Tuf and also to recombinant Tuf. Factor H and plasminogen (PLG) bound simultaneously to Tuf, and PLG was proteolytically activated.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .

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