Human Complement fragment 4a (C4a) ELISA Kit | KTE62616

Human Complement fragment 4a (C4a) ELISA Kit | KTE62616 | Gentaur UK, US & Europe Distribution

Application: This Human Complement fragment 4a (C4a) ELISA Kit employs a two-site sandwich ELISA to quantitate C4A in samples. An antibody specific for C4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Complement fragment 4a microplate
• Human Complement fragment 4a standard
• Human Complement fragment 4a detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Complement fragment 4a (C4a) ELISA Kit has high sensitivity and excellent specificity for detection of Human C4A. No significant cross-reactivity or interference between Human C4A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Complement C4-A is the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.

Alternative Names: C4A; DAMC-258G8.6; C4; C4A2; C4A3; C4A4; C4A6; C4S; CO4; CPAMD2; MGC164979; RG; C4A anaphylatoxin; Rodgers form of C4; acidic C4; complement component 4A

Search name: C4A; DAMC-258G8.6; C4; C4A2; C4A3; C4A4; C4A6; C4S; CO4; CPAMD2; MGC164979; RG; C4A anaphylatoxin; Rodgers form of C4; acidic C4; complement component 4A

Tag: C4A