Human Connective tissue-activating peptide III (CTAPIII) ELISA Kit | AE63058HU

Human Connective tissue-activating peptide III (CTAPIII) ELISA Kit | AE63058HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: CTAP?

Alternative Name: N/A

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 5.9 pg/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤7.2%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CTAP? in samples. An antibody specific for CTAP? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTAP? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTAP? is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTAP? bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Chemokine (C-X-C motif) ligand 7 (CXCL7) is a platelet-derived growth factor that belongs to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP) .Beta-thromboglobulin (β-TG) has a molecular weight of 35000 daltons and is a specific protein of platelets. Composed of 4 identical subunits; It is synthesized in the cells as a biologically inactive 15 kDa precursor called PBP (platelet basic protein) . It is stored in the Alpha-granules of platelets and released in large amounts after platelet activation.It is a protein that is released in large amounts from platelets following their activation. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .