Human Cor (Cortisol) ELISA Kit | ELK8526

Human Cor (Cortisol) ELISA Kit | ELK8526 | Gentaur UK, US & Europe Distribution

Alternative Names: Hydrocortisone

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.02 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, urine and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cortisol (Cor) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cortisol (Cor) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cortisol (Cor) in the samples is then determined by comparing the OD of the samples to the standard curve.