Human Corneodesmosin (CDSN) ELISA Kit | KTE62425

Human Corneodesmosin (CDSN) ELISA Kit | KTE62425 | Gentaur UK, US & Europe Distribution

Application: This Human Corneodesmosin (CDSN) ELISA Kit employs a two-site sandwich ELISA to quantitate CDSN in samples. An antibody specific for CDSN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCDSN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDSN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDSN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Corneodesmosin microplate
• Human Corneodesmosin standard
• Human Corneodesmosin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Corneodesmosin (CDSN) ELISA Kit has high sensitivity and excellent specificity for detection of Human CDSN. No significant cross-reactivity or interference between Human CDSN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Corneodesmosin is a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. During maturation of the cornified layers, the protein undergoes a series of cleavages, which are thought to be required for desquamation. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6.Corneodesmosomes are intercellular structures that are involved in desquamation, the shedding of superficial corneocytes from the skin surface. Corneocytes are anucleated cells derived from keratinocytes during the late stages of terminal differentiation of cornified squamous epithelia such as the epidermis.

Alternative Names: CDSN; DADB-141O4.5; D6S586E; HTSS; S; differentiated keratinocyte S protein

Search name: CDSN; DADB-141O4.5; D6S586E; HTSS; S; differentiated keratinocyte S protein

Tag: CDSN