Human CORO1A (Coronin 1A) ELISA Kit | ELK4188

Human CORO1A (Coronin 1A) ELISA Kit | ELK4188 | Gentaur UK, US & Europe Distribution

Alternative Names: CLABP; p57; CLIPINA; HCORO1; TACO; Coronin-1; Coronin, Actin-Binding Protein, 1A; Coronin-like protein A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coronin 1A (CORO1A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coronin 1A (CORO1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coronin 1A (CORO1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coronin 1A (CORO1A) in the samples is then determined by comparing the OD of the samples to the standard curve.