Human Corticosteroid Binding Globulin (CBG) ELISA Kit | AE20052HU

Human Corticosteroid Binding Globulin (CBG) ELISA Kit | AE20052HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SERPINA6

Alternative Name: CBG; corticosteroid binding globulin|serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 6|transcortin

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.073 ng/mL

Intra-Assay: ≤6.3%

Inter-Assay: ≤9.9%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SERPINA6 in samples. An antibody specific for SERPINA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Transcortin is produced by the liver and is regulated by estrogens. Therefore, plasma transcortin levels increase during pregnancy, and are decreased in cirrhosis.Transcortin is produced by the liver and is regulated by estrogens. Therefore, plasma transcortin levels increase during pregnancy, and are decreased in cirrhosis. Corticosteroid-binding globulin is an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .