Human CRAF (C-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit | ELK3763

Human CRAF (C-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit | ELK3763 | Gentaur UK, US & Europe Distribution

Alternative Names: RAF1; Raf1; V-raf-1 Murine Leukemia Viral Oncogene Homolog 1; Proto-oncogene c-RAF

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) in the samples is then determined by comparing the OD of the samples to the standard curve.