Human Creatine kinase BB isoenzyme (CK-BB) ELISA Kit | KTE62696

Human Creatine kinase BB isoenzyme (CK-BB) ELISA Kit | KTE62696 | Gentaur UK, US & Europe Distribution

Application: This Human Creatine kinase BB isoenzyme (CK-BB) ELISA Kit employs a two-site sandwich ELISA to quantitate CK-BB in samples. An antibody specific for CK-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCK-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK-BB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Creatine kinase BB isoenzyme microplate
• Human Creatine kinase BB isoenzyme standard
• Human Creatine kinase BB isoenzyme detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Creatine kinase BB isoenzyme (CK-BB) ELISA Kit has high sensitivity and excellent specificity for detection of Human CK-BB. No significant cross-reactivity or interference between Human CK-BB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Creatine kinase (CK) —also known as creatine phosphokinase (CPK) or phospho-creatine kinase—is an enzyme (EC 2.7.3.2) expressed by various tissues and cell types. CK catalyses the conversion of creatine and utilizes adenosine triphosphate (ATP) to create phosphocreatine (PCr) and adenosine diphosphate (ADP) . This CK enzyme reaction is reversible and thus ATP can be generated from PCr and ADP. In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit. Thus creatine kinase is an important enzyme in such tissues. Clinically, creatine kinase is assayed in blood tests as a marker of damage of CK-rich tissue such as in myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides, and acute kidney injury.

Alternative Names: CK-BB; CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain

Search name: CK-BB; CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain

Tag: CK-BB