Human CRISP3 (Cysteine Rich Secretory Protein 3) ELISA Kit | ELK3980

Human CRISP3 (Cysteine Rich Secretory Protein 3) ELISA Kit | ELK3980 | Gentaur UK, US & Europe Distribution

Alternative Names: CRS3; Aeg2; CRISP-3; SGP28; Specific granule protein of 28 kDa

Species: Human

Assay Type: Sandwich

Sensitivity: 0.43 ng/mL

Standard: 60 ng/mL

Detection Range: 0.94-60 ng/mL

Sample Type: Serum, plasma, saliva, sweat and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 3 (CRISP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Secretory Protein 3 (CRISP3) in the samples is then determined by comparing the OD of the samples to the standard curve.