Human CRT (Calreticulin) ELISA Kit | ELK3845

Human CRT (Calreticulin) ELISA Kit | ELK3845 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC6A8; CT1; CRTR; Sodium And Chloride Dependent Creatine Transporter 1; Solute Carrier Family 6 Member 8

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calreticulin (CRT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the OD of the samples to the standard curve.