Human CRT (Calreticulin) ELISA Kit | ELK5528

Human CRT (Calreticulin) ELISA Kit | ELK5528 | Gentaur UK, US & Europe Distribution

Alternative Names: CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Calreticulin (CRT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calreticulin (CRT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the OD of the samples to the standard curve.