Human CRYaB (Crystallin Alpha B) ELISA Kit | ELK3143

Human CRYaB (Crystallin Alpha B) ELISA Kit | ELK3143 | Gentaur UK, US & Europe Distribution

Alternative Names: CRY-AB; CRYA-B; CRYA2; CTPP2; HSPB5; Heat shock protein beta-5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component

Species: Human

Assay Type: Sandwich

Sensitivity: 0.239 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Alpha B (CRYaB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Crystallin Alpha B (CRYaB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Alpha B (CRYaB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Alpha B (CRYaB) in the samples is then determined by comparing the OD of the samples to the standard curve.