Human CRYl1 (Crystallin Lambda 1) ELISA Kit | ELK5199

Human CRYl1 (Crystallin Lambda 1) ELISA Kit | ELK5199 | Gentaur UK, US & Europe Distribution

Alternative Names: GDH; Lambda-CRY; Gul3DH; L-Gulonate 3-Dehydrogenase; Lambda-Crystallin Homolog

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Lambda 1 (CRYl1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Crystallin Lambda 1 (CRYl1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Lambda 1 (CRYl1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Lambda 1 (CRYl1) in the samples is then determined by comparing the OD of the samples to the standard curve.