Human CS (Citrate Synthase) ELISA Kit | ELK4619

Human CS (Citrate Synthase) ELISA Kit | ELK4619 | Gentaur UK, US & Europe Distribution

Alternative Names: Citrate (Si) -synthase, mitochondrial

Species: Human

Assay Type: Sandwich

Sensitivity: 2.39 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Citrate Synthase (CS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Citrate Synthase (CS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Citrate Synthase (CS) in the samples is then determined by comparing the OD of the samples to the standard curve.