Human CSF2Rb (Colony Stimulating Factor 2 Receptor Beta) ELISA Kit | ELK4301

Human CSF2Rb (Colony Stimulating Factor 2 Receptor Beta) ELISA Kit | ELK4301 | Gentaur UK, US & Europe Distribution

Alternative Names: CD131; CSF2R-B; IL3-RB; IL3RB; CSF2-RB; Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (granulocyte-Macrophage) ; GM-CSF/IL-3/IL-5 receptor common beta subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2 Receptor Beta (CSF2Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) in the samples is then determined by comparing the OD of the samples to the standard curve.