Human CTACK (Cutaneous T-Cell Attracting Chemokine) ELISA Kit | ELK1107

Human CTACK (Cutaneous T-Cell Attracting Chemokine) ELISA Kit | ELK1107 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL27; ILC; SCYA27; ALP; Skinkine; ESkine; PESKY; CTAK; Chemokine (C-C-Motif) ligand 27; Small Inducible Cytokine Subfamily A Member 27; IL-11 R-alpha-locus chemokine

Species: Human

Assay Type: Sandwich

Sensitivity: 12.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cutaneous T-Cell Attracting Chemokine (CTACK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cutaneous T-Cell Attracting Chemokine (CTACK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cutaneous T-Cell Attracting Chemokine (CTACK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cutaneous T-Cell Attracting Chemokine (CTACK) in the samples is then determined by comparing the OD of the samples to the standard curve.