Human CTLA4 (Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK1815

Human CTLA4 (Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK1815 | Gentaur UK, US & Europe Distribution

Alternative Names: CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4

Species: Human

Assay Type: Sandwich

Sensitivity: 12.4 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Immune molecule;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) in the samples is then determined by comparing the OD of the samples to the standard curve.