Human CUL9 (Cullin 9) ELISA Kit | ELK4668

Human CUL9 (Cullin 9) ELISA Kit | ELK4668 | Gentaur UK, US & Europe Distribution

Alternative Names: PARC; H7AP1; P53-Associated Parkin-Like Cytoplasmic Protein; UbcH7-associated protein 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cullin 9 (CUL9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cullin 9 (CUL9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cullin 9 (CUL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cullin 9 (CUL9) in the samples is then determined by comparing the OD of the samples to the standard curve.