Human CX3CR1 (Chemokine C-X3-C-Motif Receptor 1) ELISA Kit | ELK4545

Human CX3CR1 (Chemokine C-X3-C-Motif Receptor 1) ELISA Kit | ELK4545 | Gentaur UK, US & Europe Distribution

Alternative Names: CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28; Beta chemokine receptor-like 1; Fractalkine receptor; G-protein coupled receptor 13

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X3-C-Motif Receptor 1 (CX3CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) in the samples is then determined by comparing the OD of the samples to the standard curve.