Human Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit | KTE62006

Human Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit | KTE62006 | Gentaur UK, US & Europe Distribution

Application: This Human Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMTF1 in samples. An antibody specific for DMTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cyclin-D-binding Myb-like transcription factor 1 microplate
• Human Cyclin-D-binding Myb-like transcription factor 1 standard
• Human Cyclin-D-binding Myb-like transcription factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMTF1. No significant cross-reactivity or interference between Human DMTF1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer.

Alternative Names: DMTF1; DMP1; DMTF; FLJ25188; FLJ41265; FLJ76054; hDMP1; OTTHUMP00000205131; cyclin D-binding Myb-like protein 1

Search name: DMTF1; DMP1; DMTF; FLJ25188; FLJ41265; FLJ76054; hDMP1; OTTHUMP00000205131; cyclin D-binding Myb-like protein 1

Tag: DMTF1