Human CYP21B (Cytochrome P450 21B) ELISA Kit | ELK5576

Human CYP21B (Cytochrome P450 21B) ELISA Kit | ELK5576 | Gentaur UK, US & Europe Distribution

Alternative Names: CYP21A2; CPS1; CA21H; CAH1; CYP21; CYP21-B; 21-OHase; Steroid 21-hydroxylase; Cytochrome P450, Family 21, Subfamily A, Polypeptide 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 21B (CYP21B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 21B (CYP21B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 21B (CYP21B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 21B (CYP21B) in the samples is then determined by comparing the OD of the samples to the standard curve.