Human CYP27A1 (Cytochrome P450 27A1) ELISA Kit | ELK4688

Human CYP27A1 (Cytochrome P450 27A1) ELISA Kit | ELK4688 | Gentaur UK, US & Europe Distribution

Alternative Names: CP27; CTX; CYP27; Sterol 27-Hydroxylase, Cerebrotendinous Xanthomatosis; Vitamin D (3) 25-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the OD of the samples to the standard curve.