Human CYP2C18 (Cytochrome P450 2C18) ELISA Kit | ELK5324

Human CYP2C18 (Cytochrome P450 2C18) ELISA Kit | ELK5324 | Gentaur UK, US & Europe Distribution

Alternative Names: CYP2C; CPCI; CYP2C17; CPCJ; P450C2C; limonene 6-monooxygenase; Mephenytoin 4-hydroxylase; Cytochrome P450 Family 2 Subfamily C Polypeptide 18

Species: Human

Assay Type: Sandwich

Sensitivity: 0.264 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C18 (CYP2C18) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 2C18 (CYP2C18) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C18 (CYP2C18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2C18 (CYP2C18) in the samples is then determined by comparing the OD of the samples to the standard curve.