Human CYP2E1 (Cytochrome P450 2E1) ELISA Kit | ELK4463

Human CYP2E1 (Cytochrome P450 2E1) ELISA Kit | ELK4463 | Gentaur UK, US & Europe Distribution

Alternative Names: CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450, Subfamily IIE (Ethanol-Inducible) Polypeptide 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.32 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1 (CYP2E1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 2E1 (CYP2E1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2E1 (CYP2E1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2E1 (CYP2E1) in the samples is then determined by comparing the OD of the samples to the standard curve.