Human CYPB (Cyclophilin B) ELISA Kit | ELK2123

Human CYPB (Cyclophilin B) ELISA Kit | ELK2123 | Gentaur UK, US & Europe Distribution

Alternative Names: PPIB; PPI-B; CYP-S1; CyP-B; SCYLP; PPIase B; Rotamase B; S-cyclophilin; Peptidylprolyl Isomerase B; Peptidyl-Prolyl Cis-Trans Isomerase B

Species: Human

Assay Type: Sandwich

Sensitivity: 122 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B (CYPB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclophilin B (CYPB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin B (CYPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclophilin B (CYPB) in the samples is then determined by comparing the OD of the samples to the standard curve.