Human CYSLTR2 (Cysteinyl Leukotriene Receptor 2) ELISA Kit | ELK5345

Human CYSLTR2 (Cysteinyl Leukotriene Receptor 2) ELISA Kit | ELK5345 | Gentaur UK, US & Europe Distribution

Alternative Names: CYSLT2; GPCR; CYSLT2R; HG57; HPN321; HGPCR21; G-protein coupled receptor GPCR21; G-protein coupled receptor HG57

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 2 (CYSLTR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteinyl Leukotriene Receptor 2 (CYSLTR2) in the samples is then determined by comparing the OD of the samples to the standard curve.