Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit | AE12109HU

Human Cytochrome b-c1 complex subunit 10 (UQCR) ELISA Kit | AE12109HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UQCR

Alternative Name: 0710008D09Rik; QCR10; UQCR11; complex III subunit XI|cytochrome b-c1 complex subunit 10|ubiquinol cytochrome c reductase; 6.4kDa subunit

Application: ELISA

Range: 0.781-50 ng/mL

Sensitivity: 0.27 ng/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤8.3%

Recovery: 1, 06

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UQCR in samples. An antibody specific for UQCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR, Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site) . Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .