Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit | KTE62171

Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit | KTE62171 | Gentaur UK, US & Europe Distribution

Application: This Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP2B6 in samples. An antibody specific for CYP2B6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP2B6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2B6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2B6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytochrome P450 2B6 microplate
• Human Cytochrome P450 2B6 standard
• Human Cytochrome P450 2B6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP2B6. No significant cross-reactivity or interference between Human CYP2B6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for CYP2B6 have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

Alternative Names: CYP2B6; CPB6; CYP2B; CYPIIB6;B1; P450; cytochrome P450; subfamilyB (phenobarbital-inducible) ; polypeptide 6

Search name: CYP2B6; CPB6; CYP2B; CYPIIB6;B1; P450; cytochrome P450; subfamilyB (phenobarbital-inducible) ; polypeptide 6

Tag: CYP2B6