Human Cytochrome P450 2C19 (CYP2C19) ELISA Kit | AE58944HU

Human Cytochrome P450 2C19 (CYP2C19) ELISA Kit | AE58944HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: CYP2C19

Alternative Name: RP11-400G3.4; CPCJ; CYP2C; P450C2C; P450IIC19; S-mephenytoin 4-hydroxylase|cytochrome P-450 II C|cytochrome P450; subfamily IIC (mephenytoin 4-hydroxylase) ; polypeptide 19|flavoprotein-linked monoox

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.078 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤7.5%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CYP2C19 in samples. An antibody specific for CYP2C19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP2C19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2C19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2C19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: CYP2C19 is an enzyme. This protein, a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics, including many proton pump inhibitors and antiepileptics. CYP2C19 is a liver enzyme that acts on 10-15% of drugs in current clinical use, including the antiplatelet clopidogrel, drugs that treat pain associated with ulcers, such as omeprazole, antiseizure drugs such as mephenytoin, the antimalarial proguanil, and the anxiolytic diazepam. The gene encodes a member of the cytochrome P450 superfamily of enzymes. These proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many drugs.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .