Human Cytoglobin (CYGB) ELISA Kit | KTE62175

Human Cytoglobin (CYGB) ELISA Kit | KTE62175 | Gentaur UK, US & Europe Distribution

Application: This Human Cytoglobin (CYGB) ELISA Kit employs a two-site sandwich ELISA to quantitate CYGB in samples. An antibody specific for CYGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYGB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYGB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytoglobin microplate
• Human Cytoglobin standard
• Human Cytoglobin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytoglobin (CYGB) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYGB. No significant cross-reactivity or interference between Human CYGB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CYGB encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. CYGB is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation.

Alternative Names: CYGB; HGB; STAP; histoglobin; stellate cell activation-associated protein

Search name: CYGB; HGB; STAP; histoglobin; stellate cell activation-associated protein

Tag: CYGB