Human Cytokeratin 19 (CK-19) ELISA Kit | KTE62240

Human Cytokeratin 19 (CK-19) ELISA Kit | KTE62240 | Gentaur UK, US & Europe Distribution

Application: This Human Cytokeratin 19 (CK-19) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT19 in samples. An antibody specific for KRT19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytokeratin 19 microplate
• Human Cytokeratin 19 standard
• Human Cytokeratin 19 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytokeratin 19 (CK-19) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT19. No significant cross-reactivity or interference between Human KRT19 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Keratin, type I cytoskeletal 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.Keratin 19 is a type I cytokeratin.

Alternative Names: KRT19; CK19; K19; K1CS; MGC15366; 40-kDa keratin intermediate filament; cytokeratin 19; keratin; type I cytoskeletal 19; keratin; type I; 40-kd

Search name: KRT19; CK19; K19; K1CS; MGC15366; 40-kDa keratin intermediate filament; cytokeratin 19; keratin; type I cytoskeletal 19; keratin; type I; 40-kd

Tag: KRT19