Human Cytokeratin 20 (CK20) ELISA Kit | KTE61880

Human Cytokeratin 20 (CK20) ELISA Kit | KTE61880 | Gentaur UK, US & Europe Distribution

Application: This Human Cytokeratin 20 (CK20) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT20 in samples. An antibody specific for KRT20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytokeratin 20 microplate
• Human Cytokeratin 20 standard
• Human Cytokeratin 20 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytokeratin 20 (CK20) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT20. No significant cross-reactivity or interference between Human KRT20 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cytokeratin 20 (CK20) is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium. CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin.

Alternative Names: KRT20; CD20; CK20; K20; KRT21; MGC35423; cytokeratin 20; keratin; type I cytoskeletal 20

Search name: KRT20; CD20; CK20; K20; KRT21; MGC35423; cytokeratin 20; keratin; type I cytoskeletal 20

Tag: KRT20