Human Cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit | KTE62176

Human Cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit | KTE62176 | Gentaur UK, US & Europe Distribution

Application: This Human Cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYFRA21-1 in samples. An antibody specific for CYFRA21-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYFRA21-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYFRA21-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYFRA21-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytokeratin fragment antigen 21-1 microplate
• Human Cytokeratin fragment antigen 21-1 standard
• Human Cytokeratin fragment antigen 21-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYFRA21-1. No significant cross-reactivity or interference between Human CYFRA21-1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cytokeratin19 Fragment Antigen 21-1 (CYFRA21-1) belongs to a family of 20 related polypeptides that are constituents of the intermediate filaments of epithelial cells. Of these CK19 is the most frequently expressed cytokeratin endometrial cancer. CYFRA21-1 is a common tumor marker of lung cancer, non-small-cell cancer, small cell carcinoma and squamous cell carcinoma. For breast cancer, CYFRA21-1 may be comparable to the sensitivity and specificity of CA153. Measured jointly with any of the tumor markers as CEA, SCCA, NSE, can increase the sensitivity. Other as bladder cancer, nasopharyngeal cancer, ovarian cancer, gastrointestinal cancer, and also have certain positive rate. CYFRA21-1 in hepatitis, pancreatitis, pneumonia, benign prostatic hyperplasia may also be increased.

Alternative Names: CYFRA21-1

Search name: CYFRA21-1

Tag: CYFRA21-1