Human Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) ELISA Kit | KTE61965

Human Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) ELISA Kit | KTE61965 | Gentaur UK, US & Europe Distribution

Application: This Human Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) ELISA Kit employs a two-site sandwich ELISA to quantitate DYNC1H1 in samples. An antibody specific for DYNC1H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYNC1H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYNC1H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYNC1H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytoplasmic dynein 1 heavy chain 1 microplate
• Human Cytoplasmic dynein 1 heavy chain 1 standard
• Human Cytoplasmic dynein 1 heavy chain 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DYNC1H1. No significant cross-reactivity or interference between Human DYNC1H1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. Cytoplasmic dynein has been implicated in a variety of other forms of intracellular motility, including retrograde axonal transport, protein sorting between apical and basolateral surfaces, and redistribution of organelles like endosomes and lysosomes. The predicted partial protein sequence shares 99% and 34% identity with rat DHC1a and human DNHC2, respectively. Antibodies against DHC1 recognized a high molecular mass protein on Western blots of extracts from several mammalian cell lines. Northern blot analysis revealed that DHC1 is expressed as an approximately 15-kb mRNA in several mammalian cells lines and human tissues, including those that make neither cilia nor flagella.

Alternative Names: DYNC1H1; DHC1; DHC1a; DKFZp686P2245; DNCH1; DNCL; DNECL; DYHC; Dnchc1; HL-3; KIAA0325; p22; cytoplasmic dynein 1 heavy chain 1; dynein heavy chain; cytosolic; dynein; cytoplasmic; heavy polypeptide 1; dynein; c

Search name: DYNC1H1; DHC1; DHC1a; DKFZp686P2245; DNCH1; DNCL; DNECL; DYHC; Dnchc1; HL-3; KIAA0325; p22; cytoplasmic dynein 1 heavy chain 1; dynein heavy chain; cytosolic; dynein; cytoplasmic; heavy polypeptide 1; dynein; c

Tag: DYNC1H1