Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit | KTE61964

Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit | KTE61964 | Gentaur UK, US & Europe Distribution

Application: This Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit employs a two-site sandwich ELISA to quantitate DYNC2H1 in samples. An antibody specific for DYNC2H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYNC2H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYNC2H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYNC2H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cytoplasmic dynein 2 heavy chain 1 microplate
• Human Cytoplasmic dynein 2 heavy chain 1 standard
• Human Cytoplasmic dynein 2 heavy chain 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DYNC2H1. No significant cross-reactivity or interference between Human DYNC2H1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DYNC2H1 encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. The light intermediate chains, with molecular masses of 55 to 59 kD, are unique to cytoplasmic dynein and contain consensus ATPase elements. Both axonemal and cytoplasmic dyneins also contain light chains of 8 to 25 kD.

Alternative Names: DYNC2H1; ATD3; DHC1b; DHC2; DNCH2; DYH1B; FLJ11756; hdhc11; DYNC2H1 variant protein; cytoplasmic dynein 2 heavy chain; dynein heavy chain; isotype 1B; dynein; cytoplasmic; heavy chain-2; dynein; cytoplasmic; hea

Search name: DYNC2H1; ATD3; DHC1b; DHC2; DNCH2; DYH1B; FLJ11756; hdhc11; DYNC2H1 variant protein; cytoplasmic dynein 2 heavy chain; dynein heavy chain; isotype 1B; dynein; cytoplasmic; heavy chain-2; dynein; cytoplasmic; hea

Tag: DYNC2H1