Human DBI (Diazepam Binding Inhibitor) ELISA Kit | ELK4965

Human DBI (Diazepam Binding Inhibitor) ELISA Kit | ELK4965 | Gentaur UK, US & Europe Distribution

Alternative Names: ACBP; ACBD1; CCK-RP; EP; Endozepine; GABA Receptor Modulator; Acyl-Coenzyme A Binding Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diazepam Binding Inhibitor (DBI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diazepam Binding Inhibitor (DBI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diazepam Binding Inhibitor (DBI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diazepam Binding Inhibitor (DBI) in the samples is then determined by comparing the OD of the samples to the standard curve.