Human DBNL (Drebrin Like Protein) ELISA Kit | ELK7533

Human DBNL (Drebrin Like Protein) ELISA Kit | ELK7533 | Gentaur UK, US & Europe Distribution

Alternative Names: ABP1; HIP-55; SH3P7; CMAP; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; SH3 domain-containing protein 7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.63 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Drebrin Like Protein (DBNL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Drebrin Like Protein (DBNL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Drebrin Like Protein (DBNL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Drebrin Like Protein (DBNL) in the samples is then determined by comparing the OD of the samples to the standard curve.