Human DDR2 (Discoidin Domain Receptor Family, Member 2) ELISA Kit | ELK5000

Human DDR2 (Discoidin Domain Receptor Family, Member 2) ELISA Kit | ELK5000 | Gentaur UK, US & Europe Distribution

Alternative Names: CD167b; MIG20a; NTRKR3; TKT; TYRO10; Discoidin Domain Receptor Tyrosine Kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT

Species: Human

Assay Type: Sandwich

Sensitivity: 0.54 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discoidin Domain Receptor Family, Member 2 (DDR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discoidin Domain Receptor Family, Member 2 (DDR2) in the samples is then determined by comparing the OD of the samples to the standard curve.