Human Death-inducer obliterator 1 (DIDO1) ELISA Kit | KTE62057

Human Death-inducer obliterator 1 (DIDO1) ELISA Kit | KTE62057 | Gentaur UK, US & Europe Distribution

Application: This Human Death-inducer obliterator 1 (DIDO1) ELISA Kit employs a two-site sandwich ELISA to quantitate DIDO1 in samples. An antibody specific for DIDO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIDO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIDO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIDO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Death-inducer obliterator 1 microplate
• Human Death-inducer obliterator 1 standard
• Human Death-inducer obliterator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Death-inducer obliterator 1 (DIDO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DIDO1. No significant cross-reactivity or interference between Human DIDO1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene（DIDO1） is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for DIDO1, encoding multiple isoforms.

Alternative Names: DIDO1; RP5-885L7.1; BYE1; C20orf158; DATF1; DIDO2; DIDO3; DIO-1; DIO1; DKFZp434P1115; FLJ11265; KIAA0333; MGC16140; dJ885L7.8; OTTHUMP00000031519; OTTHUMP00000031521; OTTHUMP00000031522; death associated tran

Search name: DIDO1; RP5-885L7.1; BYE1; C20orf158; DATF1; DIDO2; DIDO3; DIO-1; DIO1; DKFZp434P1115; FLJ11265; KIAA0333; MGC16140; dJ885L7.8; OTTHUMP00000031519; OTTHUMP00000031521; OTTHUMP00000031522; death associated tran

Tag: DIDO1