Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit | KTE61990

Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit | KTE61990 | Gentaur UK, US & Europe Distribution

Application: This Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit employs a two-site sandwich ELISA to quantitate DOCK4 in samples. An antibody specific for DOCK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOCK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOCK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOCK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dedicator of cytokinesis protein 4 microplate
• Human Dedicator of cytokinesis protein 4 standard
• Human Dedicator of cytokinesis protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dedicator of cytokinesis protein 4 (DOCK4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DOCK4. No significant cross-reactivity or interference between Human DOCK4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dock4 is part of a large class of proteins (GEFs) which contibrute to cellular signalling events by activating small G proteins. In their resting state G proteins are bound to Guanosine diphosphate (GDP) and their activation requires the dissociation of GDP and binding of guanosine triphosphate (GTP) . GEFs activate G proteins by promoting this nucleotide exchange.The domain arrangement of Dock4 is largely equivalent to that of Dock180 (the archetypal member of the DOCK family) and other DOCK-A/B family members (35% sequence identity with Dock180, 39% with Dock2 and 54% with Dock3) . Dock4, however, contains a unique set of motifs at its proline-rich C-terminus which include a Src-binding site that is shared with CED-5, the C. elegans ortholog of mammalian DOCK proteins.

Alternative Names: DOCK4; WUGSC:H_GS034D21.1; FLJ34238; KIAA0716; MGC134911; MGC134912; OTTHUMP00000207227

Search name: DOCK4; WUGSC:H_GS034D21.1; FLJ34238; KIAA0716; MGC134911; MGC134912; OTTHUMP00000207227

Tag: DOCK4