Human DEFB103B (Defensin Beta 103B) ELISA Kit | ELK6584

Human DEFB103B (Defensin Beta 103B) ELISA Kit | ELK6584 | Gentaur UK, US & Europe Distribution

Alternative Names: HBP3; HBD3; BD3; DEFB103; DEFB3; Defensin-like protein; Beta-defensin 3

Species: Human

Assay Type: Sandwich

Sensitivity: 12.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the OD of the samples to the standard curve.