Human DEFb126 (Defensin Beta 126) ELISA Kit | ELK6682

Human DEFb126 (Defensin Beta 126) ELISA Kit | ELK6682 | Gentaur UK, US & Europe Distribution

Alternative Names: ESP13.2; C20orf8; DEFB-26; Epididymal secretory protein 13.2

Species: Human

Assay Type: Sandwich

Sensitivity: 3 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the OD of the samples to the standard curve.